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Duration-dependent influence of dynamic torsion on the intervertebral disc: an intact disc organ culture study

Chan, Samantha ; Walser, Jochen ; Ferguson, Stephen ; Gantenbein, Benjamin

In: European Spine Journal, 2015, vol. 24, no. 11, p. 2402-2410

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    Titel
    • Duration-dependent influence of dynamic torsion on the intervertebral disc: an intact disc organ culture study
    Autor
    • Chan, Samantha. Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Stauffacherstrasse 78, 3014, Bern, Switzerland
    • Walser, Jochen. Institute for Biomechanics, ETH Zürich, Zurich, Switzerland
    • Ferguson, Stephen. Institute for Biomechanics, ETH Zürich, Zurich, Switzerland
    • Gantenbein, Benjamin. Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Stauffacherstrasse 78, 3014, Bern, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • European Spine Journal, 2015, vol. 24, no. 11, p. 2402-2410. Springer Berlin Heidelberg
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:332078
    Summary
    Purpose: Mechanical loading is an important parameter that alters the homeostasis of the intervertebral disc (IVD). Studies have demonstrated the role of compression in altering the cellular metabolism, anabolic and catabolic events of the disc, but little is known how complex loading such as torsion-compression affects the IVD cell metabolism and matrix homeostasis. Studying how the duration of torsion affects disc matrix turnover could provide guidelines to prevent overuse injury to the disc and suggest possible beneficial effect of torsion. The aim of the study was to evaluate the biological response of the IVD to different durations of torsional loading. Methods: Intact bovine caudal IVD were isolated for organ culture in a bioreactor. Different daily durations of torsion were applied over 7days at a physiological magnitude (±2°) in combination with 0.2MPa compression, at a frequency of 1Hz. Results: Nucleus pulpous (NP) cell viability and total disc volume decreased with 8h of torsion-compression per day. Gene expression analysis suggested a down-regulated MMP13 with increased time of torsion. 1 and 4h per day torsion-compression tended to increase the glycosaminoglycans/hydroxyproline ratio in the NP tissue group. Conclusions: Our result suggests that load duration thresholds exist in both torsion and compression with an optimal load duration capable of promoting matrix synthesis and overloading can be harmful to disc cells. Future research is required to evaluate the specific mechanisms for these observed effects.