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Red Blood Cell Aging During Storage, Studied Using Optical Tweezers Experiment

Czerwinska, Justyna ; Wolf, Stefan ; Mohammadi, Hanieh ; Jeney, Sylvia

In: Cellular and Molecular Bioengineering, 2015, vol. 8, no. 2, p. 258-266

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    Titre
    • Red Blood Cell Aging During Storage, Studied Using Optical Tweezers Experiment
    Auteur
    • Czerwinska, Justyna. Artorg Center for Biomedical Engineering and Department of Nephrology, Hypertension and Clinical Pharmacology, Bern University, Murtenstrasse 50, 3010, Bern, Switzerland
    • Wolf, Stefan. Artorg Center for Biomedical Engineering and Department of Nephrology, Hypertension and Clinical Pharmacology, Bern University, Murtenstrasse 50, 3010, Bern, Switzerland
    • Mohammadi, Hanieh. Artorg Center for Biomedical Engineering and Department of Nephrology, Hypertension and Clinical Pharmacology, Bern University, Murtenstrasse 50, 3010, Bern, Switzerland
    • Jeney, Sylvia. Laboratory of Nanostructures and Novel Electronic Materials, EPFL SB-IPMC, Station 3, Bâtiment PH, 1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Cellular and Molecular Bioengineering, 2015, vol. 8, no. 2, p. 258-266. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:331230
    Summary
    This paper presents experimental and numerical studies of erythrocyte stretching, with a focus on the aging of red blood cells in an in vitro environment during storage. The experimental studies were performed using optical tweezers. The laser beam was used to pull and stretch a cell sedimented on a flat surface. A force calibration was obtained via a comparison of the experimental data with results from finite element simulations of the cell stretching. The experiments were performed using blood samples from blood bank donations made by three donors. The experiments were performed over 21days of storage, and the estimate erythrocyte membrane shear modulus during this period increased from 2.5 to 13μN/m.