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Development and validation of a rapid turboflow LC-MS/MS method for the quantification of LSD and 2-oxo-3-hydroxy LSD in serum and urine samples of emergency toxicological cases

Dolder, Patrick ; Liechti, Matthias ; Rentsch, Katharina

In: Analytical and Bioanalytical Chemistry, 2015, vol. 407, no. 6, p. 1577-1584

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    Titel
    • Development and validation of a rapid turboflow LC-MS/MS method for the quantification of LSD and 2-oxo-3-hydroxy LSD in serum and urine samples of emergency toxicological cases
    Autor
    • Dolder, Patrick. Laboratory Medicine, University Hospital, Petersgraben 4, 4031, Basel, Switzerland
    • Liechti, Matthias. Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Clinical Research, University Hospital and University of Basel, Hebelstrasse 20, 4031, Basel, Switzerland
    • Rentsch, Katharina. Laboratory Medicine, University Hospital, Petersgraben 4, 4031, Basel, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Analytical and Bioanalytical Chemistry, 2015, vol. 407, no. 6, p. 1577-1584. Springer Berlin Heidelberg
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:331048
    Summary
    Lysergic acid diethylamide (LSD) is a widely used recreational drug. The aim of the present study is to develop a quantitative turboflow LC-MS/MS method that can be used for rapid quantification of LSD and its main metabolite 2-oxo-3-hydroxy LSD (O-H-LSD) in serum and urine in emergency toxicological cases without time-consuming extraction steps. The method was developed on an ion-trap LC-MS/MS instrument coupled to a turbulent-flow extraction system. The validation data showed no significant matrix effects and no ion suppression has been observed in serum and urine. Mean intraday accuracy and precision for LSD were 101 and 6.84%, in urine samples and 97.40 and 5.89% in serum, respectively. For O-H-LSD, the respective values were 97.50 and 4.99% in urine and 107 and 4.70% in serum. Mean interday accuracy and precision for LSD were 100 and 8.26% in urine and 101 and 6.56% in serum, respectively. For O-H-LSD, the respective values were 101 and 8.11% in urine and 99.8 and 8.35% in serum, respectively. The lower limit of quantification for LSD was determined to be 0.1ng/ml. LSD concentrations in serum were expected to be up to 8ng/ml. 2-Oxo-3-hydroxy LSD concentrations in urine up to 250ng/ml. The new method was accurate and precise in the range of expected serum and urine concentrations in patients with a suspected LSD intoxication. Until now, the method has been applied in five cases with suspected LSD intoxication where the intake of the drug has been verified four times with LSD concentrations in serum in the range of 1.80-14.70ng/ml and once with a LSD concentration of 1.25ng/ml in urine. In serum of two patients, the O-H-LSD concentration was determined to be 0.99 and 0.45ng/ml. In the urine of a third patient, the O-H-LSD concentration was 9.70ng/ml.