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Kinetics of microRNA Expression in Bronchoalveolar Lavage Fluid Samples

Brock, Matthias ; Rechsteiner, Thomas ; Kohler, Malcolm ; Franzen, Daniel ; Huber, Lars

In: Lung, 2015, vol. 193, no. 3, p. 381-385

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    Titre
    • Kinetics of microRNA Expression in Bronchoalveolar Lavage Fluid Samples
    Auteur
    • Brock, Matthias. Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland
    • Rechsteiner, Thomas. Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland
    • Kohler, Malcolm. Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland
    • Franzen, Daniel. Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland
    • Huber, Lars. Division of Pulmonology, University Hospital Zurich, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Lung, 2015, vol. 193, no. 3, p. 381-385. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:331025
    Summary
    Levels of microRNAs (miRNAs) are increasingly assessed in biological fluids, for example, in samples obtained by bronchoalveolar lavage (BAL). "Post-collection kinetics” of miRNA expression levels, however, have not been investigated to date. In these experiments, we analyzed the dynamic expression profile of 5 different miRNAs (miR-17, miR-19b, miR-20b, miR-125a, and miR-223-3p) in BAL within the first 24h following collection by routine bronchoscopy. miRNAs were quantified 0, 1, 4, 8, and 24h after collection in samples that were kept at 4°C or at room temperature. The expression of all five miRNAs was found to remain stable between the first 8h after collection. 24h after collection miRNAs faced substantial alterations in their expression profile. These data emphasize that BAL samples intended for further miRNA analysis can be handled at room temperature within the first 8h after bronchoscopy.