Reduction of nanoparticle load in cells by mitosis but not exocytosis
- Bourquin, Joël Adolphe Merkle Institute, University of Fribourg, Switzerland
- Septiadi, Dedy Adolphe Merkle Institute, University of Fribourg, Switzerland
- Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
- Balog, Sandor Adolphe Merkle Institute, University of Fribourg, Switzerland
- Steinmetz, Lukas Adolphe Merkle Institute, University of Fribourg, Switzerland
- Spuch-Calvar, Miguel Adolphe Merkle Institute, University of Fribourg, Switzerland
- Taladriz-Blanco, Patricia Adolphe Merkle Institute, University of Fribourg, Switzerland
- Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland
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23.07.2019
Published in:
- ACS Nano. - 2019, vol. 13, no. 7, p. 7759–7770
English
The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division, e.g., mitosis. Additionally, NP distribution after cell division is observed to be asymmetrical, likely due to the inhomogeneous location and distribution of the NP-loaded intracellular vesicles in the mother cells. These findings are important for biomedical and hazard studies as the NP load per cell can vary significantly. Furthermore, we highlight the possibility of biopersistent NP accumulation over time within the mononuclear phagocyte system.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie, AMI - Bio-Nanomatériaux
- Language
-
- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 326960
- DOI 10.1021/acsnano.9b01604
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308113
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