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Static biofilm removal around ultrasonic tips in vitro

Thurnheer, Thomas ; Rohrer, Elodie ; Belibasakis, Georgios ; Attin, Thomas ; Schmidlin, Patrick

In: Clinical Oral Investigations, 2014, vol. 18, no. 7, p. 1779-1784

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    Titre
    • Static biofilm removal around ultrasonic tips in vitro
    Auteur
    • Thurnheer, Thomas. Section for Oral Microbiology and General Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland
    • Rohrer, Elodie. Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zürich, Switzerland
    • Belibasakis, Georgios. Section for Oral Microbiology and General Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland
    • Attin, Thomas. Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zürich, Switzerland
    • Schmidlin, Patrick. Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zürich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Clinical Oral Investigations, 2014, vol. 18, no. 7, p. 1779-1784. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:325145
    Summary
    Objectives: This study aims to investigate the biofilm removal capacity of two ultrasonic tips under standardized conditions using a multi-species biofilm model. Methods: Six-species biofilms were grown on hydroxyapatite discs for 64.5h and were treated for 15s with a standardized load of 40g with a piezoelectric or magnetostrictive device. Tips were applied either with the tip end or with the side facing downwards. Detached bacteria were determined in the supernatant and colony-forming units (CFUs) counted after 72h of incubation. Untreated specimens served as controls. Moreover, the biofilms remaining on the hydroxyapatite surface after treatment were stained using the Live/Dead stain, and the pattern of their detachment was assessed by confocal laser scanning microscopy (CLSM). Results: As compared to the untreated control, it was found that only a side application of the magnetostrictive device was able to remove efficiently the biofilm. In contrast, its tip application as well as both applications of the piezoelectric device removed significantly less bacteria from the biofilm structure. These findings were corroborated by CLSM observation. Conclusion: Both ultrasonic tips under investigations led to bacterial detachment, but the action mode as well as the tip configuration and adaptation appeared to be influenced by the biofilm removal effectiveness. Clinical relevance: Biofilm removal remains a main goal of ultrasonic debridement. This should be reflected in respective laboratory investigations. The presented combination of methods applied on a multi-species biofilm model in vitro allows the evaluation of the effectiveness of different ultrasonic scaler applications.