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Evaluation of commercial slides for detection of immunoglobulin G against Bartonella henselae by indirect immunofluorescence

Zbinden, R. ; Michael, N. ; Sekulovski, M. ; von Graevenitz, A. ; Nadal, D.

In: European Journal of Clinical Microbiology and Infectious Diseases, 1997, vol. 16, no. 9, p. 648-652

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    Title
    • Evaluation of commercial slides for detection of immunoglobulin G against Bartonella henselae by indirect immunofluorescence
    Author
    • Zbinden, R.. Department of Medical Microbiology, University of Zurich, Gloriastrasse 32, CH-8028, Zurich, Switzerland
    • Michael, N.. Department of Medical Microbiology, University of Zurich, Gloriastrasse 32, CH-8028, Zurich, Switzerland
    • Sekulovski, M.. Department of Medical Microbiology, University of Zurich, Gloriastrasse 32, CH-8028, Zurich, Switzerland
    • von Graevenitz, A.. Department of Medical Microbiology, University of Zurich, Gloriastrasse 32, CH-8028, Zurich, Switzerland
    • Nadal, D.. Infectious Diseases Unit, University Children's Hospital, CH-8032, Zurich, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • European Journal of Clinical Microbiology and Infectious Diseases, 1997, vol. 16, no. 9, p. 648-652. Springer-Verlag
    Other Version
    Classification
    • Health
    OAI-PMH Identifier
    • oai:doc.rero.ch:322317
    Summary
    Four commercial slides were compared with in-house slides for the detection of immunoglobulin G (IgG) againstBartonella henselae in 58 healthy persons from a rural region by an indirect immunofluorescence assay. MRL-BA slides (MRL Diagnostics, USA) and Virion slides (Virion, Switzerland) with agar-derivedBartonella henselae showed IgG titers of ≥ 1∶256 in 44.8% and 51.7%, respectively, whereas Bion slides (Bios, Germany), MRL-Vero slides (MRL Diagnostics), and in-house slides with cell-associatedBartonella henselae showed such titers in 3.4%, 5.1% and 3.4%, respectively. The MRL-Vero slides (Bartonella IgG substrate slides, MRL Diagnostics) were further evaluated with 26 patients with cat scratch disease, 20 patients with lymphadenopathy not due to cat scratch disease, 100 blood donors from an urban area, and 120 blood donors from a mixed urban/rural area. In our mixed urban/rural population the IgG titer of 1∶256 had a sensitivity of 84.6% and a specificity of 93.4% for the serodiagnosis of cat scratch disease. Seroprevalence was higher in blood donors from the mixed area (50.8%) than from the urban area (37%). MRL-Vero slides were considered useful for the serodiagnosis of cat scratch disease by indirect immunofluorescence and have replaced our in-house system. However, patients with low IgG titers should be retested three to four weeks after initial sampling to demonstrate a possible rise of IgG titers in paired sera