Fulltext

New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

Guillarme, Davy ; Ruta, Josephine ; Rudaz, Serge ; Veuthey, Jean-Luc

In: Analytical and Bioanalytical Chemistry, 2010, vol. 397, no. 3, p. 1069-1082

Aggiungi alla tua lista
    Titre
    • New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches
    Auteur
    • Guillarme, Davy. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211, Geneva 4, Switzerland
    • Ruta, Josephine. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211, Geneva 4, Switzerland
    • Rudaz, Serge. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211, Geneva 4, Switzerland
    • Veuthey, Jean-Luc. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211, Geneva 4, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2010, vol. 397, no. 3, p. 1069-1082. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:322211
    Summary
    Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic supports, elevated mobile phase temperatures, and columns packed with sub-3μm superficially porous particles (fused core) or with sub-2μm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular weights ranging between 200 and 1300g mol−1 in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature is useful for larger molecules, for example peptides. Figure Recent progress in HPLC technology to increase throughput and resolving power