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Cell-free foetal DNA in maternal plasma does not appear to be derived from the rich pool of cell-free foetal DNA in amniotic fluid

Zhong, Xiao ; Holzgreve, Wolfgang ; Tercanli, Sevgi ; Wenzel, Friedel ; Hahn, Sinuhe

In: Archives of Gynecology and Obstetrics, 2006, vol. 273, no. 4, p. 221-226

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    Title
    • Cell-free foetal DNA in maternal plasma does not appear to be derived from the rich pool of cell-free foetal DNA in amniotic fluid
    Author
    • Zhong, Xiao. Department of Research,Laboratory for Prenatal Medicine, University Women's Hospital, Spitalstrasse 21, 4031, Basel, Switzerland
    • Holzgreve, Wolfgang. Department of Research,Laboratory for Prenatal Medicine, University Women's Hospital, Spitalstrasse 21, 4031, Basel, Switzerland
    • Tercanli, Sevgi. University Women's Hospital, Basel, Switzerland
    • Wenzel, Friedel. Department of Medical Genetics, University Hospital, Basel, Switzerland
    • Hahn, Sinuhe. Department of Research,Laboratory for Prenatal Medicine, University Women's Hospital, Spitalstrasse 21, 4031, Basel, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Archives of Gynecology and Obstetrics, 2006, vol. 273, no. 4, p. 221-226. Springer-Verlag
    Other Version
    Classification
    • Health
    OAI-PMH Identifier
    • oai:doc.rero.ch:321097
    Summary
    Background: Large quantities of cell-free foetal DNA have been detected in amniotic fluid, and it has been proposed that this material may contribute to the pool of cell-free foetal DNA in maternal plasma. Methods: Twelve maternal blood samples were obtained from pregnant women about to undergo an amniocentesis. Cell-free DNA was extracted from the maternal plasma samples and the matched amniotic fluid samples. The amount of cell-free foetal DNA was quantified by real-time PCR assays for the SRY and RHD genes. Results: Amniotic fluid was found to contain vast quantities of cell-free DNA (median concentration = 3,978 copies/ml amniotic fluid). The concentration of cell-free foetal DNA in maternal plasma was much lower (median concentration = 96.6 copies/ml maternal plasma). No significant correlation could, however, be determined between these two pools of cell-free foetal DNA. Conclusions: Our data confirm that amniotic fluid contains prodigious quantities of cell-free foetal DNA, but as no relationship exists between this material and that in the maternal circulation, it is unlikely that the amnion contributes to the presence of cell-free foetal DNA in maternal plasma