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Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration

Porta, Francesca ; Bracht, Hendrik ; Weikert, Christian ; Beck, Mario ; Takala, Jukka ; Brandt, Sebastian ; Hiltebrand, Luzius ; Jakob, Stephan

In: Inflammation, 2009, vol. 32, no. 5, p. 315-321

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    Titre
    • Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration
    Auteur
    • Porta, Francesca. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Bracht, Hendrik. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Weikert, Christian. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Beck, Mario. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Takala, Jukka. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Brandt, Sebastian. Department of Anesthesiology and Pain Therapy, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Hiltebrand, Luzius. Department of Anesthesiology and Pain Therapy, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    • Jakob, Stephan. Department of Intensive Care Medicine, Inselspital, Bern University Hospital and University of Bern, 3010, Freiburgstrasse, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Inflammation, 2009, vol. 32, no. 5, p. 315-321. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320930
    Summary
    Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro