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Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

Gygax, M. ; Gianfranceschi, L. ; Liebhard, R. ; Kellerhals, M. ; Gessler, C. ; Patocchi, A.

In: Theoretical and Applied Genetics, 2004, vol. 109, no. 8, p. 1702-1709

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    Titel
    • Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii
    Autor
    • Gygax, M.. Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    • Gianfranceschi, L.. Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    • Liebhard, R.. Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    • Kellerhals, M.. Agroscope FAW Wädenswil, 8820, Wädenswil, Switzerland
    • Gessler, C.. Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    • Patocchi, A.. Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zurich, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Theoretical and Applied Genetics, 2004, vol. 109, no. 8, p. 1702-1709. Springer-Verlag; http://www.springer.de
    Andere elektronische Ausgabe
    Klassifikation
    • Landwirtschaft, Agronomie
    OAI-PMH-ID
    • oai:doc.rero.ch:320893
    Summary
    Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance gene Vbj originating from the crab apple Malus baccata jackii. Using bulk segregant analysis, three RAPD markers linked to Vbj were first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be co-dominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype-phenotype incongruence (GPI plants) plants, a linkage map was calculated. Vbj mapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6cM from CH05e03 and at 3.9cM from T6-SCAR. Without the removal of the GPI plants, Vbj was placed 15cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated