Interfacing liquid chromatography with atmospheric pressure MALDI-MS
Daniel, Jürg ; Laiko, Victor ; Doroshenko, Vladimir ; Zenobi, Renato
In: Analytical and Bioanalytical Chemistry, 2005, vol. 383, no. 6, p. 895-902
Aggiungi alla tua lista- Summary
- Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3fmol using a solution of 50nM peptide with 25mM α-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method