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Interfacing liquid chromatography with atmospheric pressure MALDI-MS

Daniel, Jürg ; Laiko, Victor ; Doroshenko, Vladimir ; Zenobi, Renato

In: Analytical and Bioanalytical Chemistry, 2005, vol. 383, no. 6, p. 895-902

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    Titre
    • Interfacing liquid chromatography with atmospheric pressure MALDI-MS
    Auteur
    • Daniel, Jürg. Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, (ETH),Hönggerberg, 8093, Zürich, Switzerland
    • Laiko, Victor. MassTech Inc., 6992 Columbia Gateway Drive, 21046, Columbia, MD, USA
    • Doroshenko, Vladimir. MassTech Inc., 6992 Columbia Gateway Drive, 21046, Columbia, MD, USA
    • Zenobi, Renato. Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, (ETH),Hönggerberg, 8093, Zürich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2005, vol. 383, no. 6, p. 895-902. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320631
    Summary
    Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3fmol using a solution of 50nM peptide with 25mM α-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method