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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

Baldi, Lucia ; Hacker, David ; Adam, Myriam ; Wurm, Florian

In: Biotechnology Letters, 2007, vol. 29, no. 5, p. 677-684

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    Titre
    • Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives
    Auteur
    • Baldi, Lucia. Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    • Hacker, David. Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    • Adam, Myriam. Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    • Wurm, Florian. Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Biotechnology Letters, 2007, vol. 29, no. 5, p. 677-684. Kluwer Academic Publishers
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320617
    Summary
    The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gene expression (TGE) is addressing this factor in an impressive way: following DNA transfection, the production phase for TGE is usually 2-10days. Recombinant proteins (r-proteins) produced by TGE can therefore enter the drug development and screening process in a very short time--weeks. With "classical” approaches to protein expression from mammalian cells, it takes months to establish a productive host cell line. This article summarizes efforts in industry and academia to use TGE to produce tens to hundreds of milligrams of r-proteins for either fundamental research or preclinical studies