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Rapid analysis of fluoxetine and its metabolite in plasma by LC-MS with column-switching approach

Souverain, S. ; Mottaz, M. ; Cherkaoui, S. ; Veuthey, J.-L

In: Analytical and Bioanalytical Chemistry, 2003, vol. 377, no. 5, p. 880-885

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    Titre
    • Rapid analysis of fluoxetine and its metabolite in plasma by LC-MS with column-switching approach
    Auteur
    • Souverain, S.. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmacy, University of Geneva, 20 bvd d'Yvoy, 1211, Geneva 4, Switzerland
    • Mottaz, M.. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmacy, University of Geneva, 20 bvd d'Yvoy, 1211, Geneva 4, Switzerland
    • Cherkaoui, S.. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmacy, University of Geneva, 20 bvd d'Yvoy, 1211, Geneva 4, Switzerland
    • Veuthey, J.-L. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmacy, University of Geneva, 20 bvd d'Yvoy, 1211, Geneva 4, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2003, vol. 377, no. 5, p. 880-885. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320289
    Summary
    Abstract.: A rapid and sensitive method was developed for the simultaneous determination of fluoxetine and its primary metabolite, norfluoxetine, in plasma. It was based on a column-switching approach with a precolumn packed with large size particles coupled with a liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS). After a simple centrifugation, plasma samples were directly injected onto the precolumn. The endogenous material was excluded thanks to a high flow rate while analytes were retained by hydrophobic interactions. Afterwards, the target compounds were eluted in back flush mode to an octadecyl analytical column and detected by ESI-MS. The overall analysis time per sample, from plasma sample preparation to data acquisition, was achieved in less than 4min. Method performances were evaluated. The method showed good linearity in the range of 25-1000ngmL−1 with a determination coefficient higher than 0.99. Limits of quantification were estimated at 25ngmL−1 for fluoxetine and norfluoxetine. Moreover, method precision was better than 6% in the studied concentration range. These results demonstrated that the method could be used to quantify target compounds. Finally, the developed assay proved to be suitable for the simultaneous analysis of fluoxetine and its metabolite in real plasma samples