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The Igf2as Transcript is Exported into Cytoplasm and Associated with Polysomes

Duart-Garcia, Carolina ; Braunschweig, Martin

In: Biochemical Genetics, 2013, vol. 51, no. 1-2, p. 119-130

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    Title
    • The Igf2as Transcript is Exported into Cytoplasm and Associated with Polysomes
    Author
    • Duart-Garcia, Carolina. Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001, Berne, Switzerland
    • Braunschweig, Martin. Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001, Berne, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Biochemical Genetics, 2013, vol. 51, no. 1-2, p. 119-130. Springer US; http://www.springer-ny.com
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:320157
    Summary
    Murine insulin-like growth factor 2 antisense (Igf2as) transcripts originate from the opposite strand of the same Igf2 locus as the Igf2 sense mRNA. The Igf2, insulin 2 (Ins2), and H19 genes form a cluster of imprinted genes on chromosome 7. Loss of imprinting of IGF2 in humans is associated with Beckwith-Wiedemann syndrome and Silver-Russell syndrome, as well as with Wilm's tumor and colorectal cancer. We developed a RNA-FISH protocol to detect Igf2as and Igf2 transcripts. The results from the RNA-FISH were confirmed with quantitative real-time PCR and clearly indicate that the Igf2as transcripts are predominantly located in the cytoplasm of C2C12 cells. In a polysome association study, we showed that the Igf2as sedimented with polysomes in a sucrose gradient. The cellular localization of Igf2as transcripts together with polysome fractionation analysis provides compelling evidence that the Igf2as is protein coding