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Performance and microbial community composition dynamics of aerobic granular sludge from sequencing batch bubble column reactors operated at 20°C, 30°C, and 35°C

Ebrahimi, Sirous ; Gabus, Sébastien ; Rohrbach-Brandt, Emmanuelle ; Hosseini, Maryam ; Rossi, Pierre ; Maillard, Julien ; Holliger, Christof

In: Applied Microbiology and Biotechnology, 2010, vol. 87, no. 4, p. 1555-1568

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    Titre
    • Performance and microbial community composition dynamics of aerobic granular sludge from sequencing batch bubble column reactors operated at 20°C, 30°C, and 35°C
    Auteur
    • Ebrahimi, Sirous. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Gabus, Sébastien. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Rohrbach-Brandt, Emmanuelle. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Hosseini, Maryam. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Rossi, Pierre. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Maillard, Julien. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    • Holliger, Christof. Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory for Environmental Biotechnology, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Applied Microbiology and Biotechnology, 2010, vol. 87, no. 4, p. 1555-1568. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320098
    Summary
    Two bubble column sequencing batch reactors fed with an artificial wastewater were operated at 20°C, 30°C, and 35°C. In a first stage, stable granules were obtained at 20°C, whereas fluffy structures were observed at 30°C. Molecular analysis revealed high abundance of the operational taxonomic unit 208 (OTU 208) affiliating with filamentous bacteria Leptothrix spp. at 30°C, an OTU much less abundant at 20°C. The granular sludge obtained at 20°C was used for the second stage during which one reactor was maintained at 20°C and the second operated at 30°C and 35°C after prior gradual increase of temperature. Aerobic granular sludge with similar physical properties developed in both reactors but it had different nutrient elimination performances and microbial communities. At 20°C, acetate was consumed during anaerobic feeding, and biological phosphorous removal was observed when Rhodocyclaceae-affiliating OTU 214 was present. At 30°C and 35°C, acetate was mainly consumed during aeration and phosphorous removal was insignificant. OTU 214 was almost absent but the Gammaproteobacteria-affiliating OTU 239 was more abundant than at 20°C. Aerobic granular sludge at all temperatures contained abundantly the OTUs 224 and 289 affiliating with Sphingomonadaceae indicating that this bacterial family played an important role in maintaining stable granular structures