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Granulocyte Colony-stimulating Factor Supports Liver Regeneration in a Small-for-size Liver Remnant Mouse Model

Inderbitzin, Daniel ; Beldi, Guido ; Sidler, Daniel ; Studer, Peter ; Keogh, Adrian ; Bisch-Knaden, Sonja ; Weimann, Rosy ; Kappeler, Andreas ; Gloor, Beat ; Candinas, Daniel

In: Journal of Gastrointestinal Surgery, 2007, vol. 11, no. 3, p. 280-285

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    Title
    • Granulocyte Colony-stimulating Factor Supports Liver Regeneration in a Small-for-size Liver Remnant Mouse Model
    Author
    • Inderbitzin, Daniel. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Beldi, Guido. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Sidler, Daniel. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Studer, Peter. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Keogh, Adrian. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Bisch-Knaden, Sonja. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Weimann, Rosy. Department of Pathology, University Hospital Bern, 3010, Bern, Switzerland
    • Kappeler, Andreas. Department of Pathology, University Hospital Bern, 3010, Bern, Switzerland
    • Gloor, Beat. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    • Candinas, Daniel. Department of Visceral and Transplantation Surgery, University Hospital Bern, 3010, Bern, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:319614
    Summary
    Experimental partial hepatectomy of more than 80% of the liver weight bears an increased mortality in rodents, due to impaired hepatic regeneration in small-for-size liver remnants. Granulocyte colony-stimulating factor (G-CSF) promotes progenitor cell expansion and mobilization and also has immunomodulatory properties. The aim of this study was to determine the effect of systemically administered G-CSF on liver regeneration and animal survival in a small-for-size liver remnant mouse model. Mice were preconditioned daily for 5days with subcutaneous injections of 5μg G-CSF or aqua ad injectabile. Subsequently, 83% partial hepatectomy was performed by resecting the median, the left, the caudate, and the right inferior hepatic lobes in all animals. Daily sham or G-CSF injection was continued. Survival was significantly better in G-CSF-treated animals (P < 0.0001). At 36 and 48h after microsurgical hepatic resection, markers of hepatic proliferation (Ki67, BrdU) were elevated in G-CSF-treated mice compared to sham injected control animals (P < 0.0001) and dry liver weight was increased (P < 0.05). G-CSF conditioning might prove to be useful in patients with small-for-size liver remnants after extended hepatic resections due to primary or secondary liver tumors or in the setting of split liver transplantation