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Developing Hyperpolarized 13C Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart

Weiss, Kilian ; Mariotti, Erika ; Hill, Deborah ; Orton, Matthew ; Dunn, Joel ; Medina, Rodolfo ; Southworth, Richard ; Kozerke, Sebastian ; Eykyn, Thomas

In: Applied Magnetic Resonance, 2012, vol. 43, no. 1-2, p. 275-288

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    Title
    • Developing Hyperpolarized 13C Spectroscopy and Imaging for Metabolic Studies in the Isolated Perfused Rat Heart
    Author
    • Weiss, Kilian. Institute for Biomedical Engineering, University and ETH Zurich, Gloriastrasse 35, 8092, Zurich, Switzerland
    • Mariotti, Erika. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    • Hill, Deborah. CRUK and EPSRC Cancer Imaging Centre, Royal Marsden NHS Trust, The Institute of Cancer Research, Sutton, UK
    • Orton, Matthew. CRUK and EPSRC Cancer Imaging Centre, Royal Marsden NHS Trust, The Institute of Cancer Research, Sutton, UK
    • Dunn, Joel. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    • Medina, Rodolfo. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    • Southworth, Richard. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    • Kozerke, Sebastian. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    • Eykyn, Thomas. Division of Imaging Sciences and Biomedical Engineering, King's College London, St Thomas Hospital, London, UK
    Document Type
    • Postprint
    Classification
    • Physics
    OAI-PMH Identifier
    • oai:doc.rero.ch:318928
    Summary
    Hyperpolarized 13C magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized 13C investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-13C] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production