Fulltext

Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I

Stettler, Alexandra ; Chaurin, Valérie ; Constable, Edwin ; Housecroft, Catherine ; Schwarz, Maria

In: JBIC Journal of Biological Inorganic Chemistry, 2007, vol. 12, no. 2, p. 194-203

Ajouter à la liste personnelle
    Titre
    • Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I
    Auteur
    • Stettler, Alexandra. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland
    • Chaurin, Valérie. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland
    • Constable, Edwin. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland
    • Housecroft, Catherine. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland
    • Schwarz, Maria. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • JBIC Journal of Biological Inorganic Chemistry, 2007, vol. 12, no. 2, p. 194-203. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:318250
    Summary
    The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified