Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I
Stettler, Alexandra ; Chaurin, Valérie ; Constable, Edwin ; Housecroft, Catherine ; Schwarz, Maria
In: JBIC Journal of Biological Inorganic Chemistry, 2007, vol. 12, no. 2, p. 194-203
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- The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified