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On-line electrogeneration of copper-peptide complexes in microspray mass spectrometry

Prudent, Michel ; Girault, Hubert

In: Journal of the American Society for Mass Spectrometry, 2008, vol. 19, no. 4, p. 560-568

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    Title
    • On-line electrogeneration of copper-peptide complexes in microspray mass spectrometry
    Author
    • Prudent, Michel. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    • Girault, Hubert. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Journal of the American Society for Mass Spectrometry, 2008, vol. 19, no. 4, p. 560-568. Springer-Verlag
    Other Version
    Classification
    • Chemistry
    OAI-PMH Identifier
    • oai:doc.rero.ch:318017
    Summary
    The interaction of copper ions with peptides was investigated by electrospray mass spectrometry. Two electrospray micro-emitters were compared, the first one with a platinum electrode using a copper(II) electrolyte solution containing a peptide sample, and the second one with a sacrificial copper anode in a water/methanol solution containing only a peptide (i.e., angiotensin III, bradykinin, or Leu-enkephalin). The former yielded mainly Cu2+ complexes either with histidine residues or with the peptide backbone (Cu+ complexes can be also formed due to gas-phase reactions), whereas the latter can generate a mixture of both Cu+ and Cu2+ aqueous complexes that yield different complexation patterns. This study shows that electrospray emitters with soluble copper anodes enable the study of Cu(I)-peptide complexes in solution