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Genetic and cytological characterization of the RecA-homologous proteins Rad51 and Dmc1 of Schizosaccharomyces pombe

Grishchuk, Alexandra ; Kraehenbuehl, Rolf ; Molnar, Monika ; Fleck, Oliver ; Kohli, Juerg

In: Current Genetics, 2004, vol. 44, no. 6, p. 317-328

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    Title
    • Genetic and cytological characterization of the RecA-homologous proteins Rad51 and Dmc1 of Schizosaccharomyces pombe
    Author
    • Grishchuk, Alexandra. Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    • Kraehenbuehl, Rolf. Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    • Molnar, Monika. Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    • Fleck, Oliver. Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    • Kohli, Juerg. Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012, Bern, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:317729
    Summary
    The Schizosaccharomyces pombe rad51 + and dmc1 + genes code for homologues of the Escherichia coli recombination protein RecA. Deletion of rad51 + causes slow growth, retardation of cell division and a decrease in viability. rad51Δ cells have a defect in mating-type switching. The DNA modification at the mating-type locus required for mating-type switching contributes to slow growth in the rad51 mutant. Cell mating is reduced in crosses homozygous for rad51Δ. Ectopic expression of the dmc1 + gene allowed us to demonstrate that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dmc1-rad24 bicistronic RNA. We describe the functional defects of terminally epitope-tagged Dmc1 and Rad51 and discuss it in terms of protein interaction. Presumptive Rad51 and Dmc1 foci were detected on spreads of meiotic chromatin