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Opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro

Keiser, Jennifer ; Utzinger, Jürg ; Xiao, Shu-Hua ; Odermatt, Peter ; Tesana, Smarn

In: Parasitology Research, 2008, vol. 102, no. 4, p. 771-776

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    Titre
    • Opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro
    Auteur
    • Keiser, Jennifer. Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, P.O. Box, CH-4002, Basel, Switzerland
    • Utzinger, Jürg. Department of Public Health and Epidemiology, Swiss Tropical Institute, P.O. Box, CH-4002, Basel, Switzerland
    • Xiao, Shu-Hua. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 200025, Shanghai, People's Republic of China
    • Odermatt, Peter. Department of Public Health and Epidemiology, Swiss Tropical Institute, P.O. Box, CH-4002, Basel, Switzerland
    • Tesana, Smarn. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
    Type de document
    • Postprint
    Classification
    • Biologie, Sciences de la vie
    Identifiant OAI-PMH
    • oai:doc.rero.ch:317069
    Summary
    The tegumental changes in adult Opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (SEM). We exposed O. viverrini to tribendimidine at a concentration of 10μg/ml for 4h. In addition, hamsters were treated with a single 400mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96h post-treatment. SEM analysis of the flukes incubated in vitro showed only mild damage of the tegument. Twenty-four hours post-treatment of hamsters, extensive disruption such as sloughing, furrowing, or blebbing of the tegument was evident, which did not increase in severity 48-72h post-treatment. Ninety-six hours after tribendimidine administration, the majority of flukes had been expelled. A single 400mg/kg oral dose of tribendimidine administered to O. viverrini-infected hamsters yielded a worm burden reduction of 63.0%. In conclusion, our experiments confirm that tribendimidine possesses interesting trematocidal properties, which warrant additional investigations and provide further data for subsequent clinical trials