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Rust mite resistance in apple assessed by quantitative trait loci analysis

Stoeckli, Sibylle ; Mody, Karsten ; Patocchi, Andrea ; Kellerhals, Markus ; Dorn, Silvia

In: Tree Genetics & Genomes, 2009, vol. 5, no. 1, p. 257-267

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    Titre
    • Rust mite resistance in apple assessed by quantitative trait loci analysis
    Auteur
    • Stoeckli, Sibylle. ETH Zurich, Institute of Plant Sciences/Applied Entomology, 8092, Zurich, Switzerland
    • Mody, Karsten. ETH Zurich, Institute of Plant Sciences/Applied Entomology, 8092, Zurich, Switzerland
    • Patocchi, Andrea. ETH Zurich, Institute of Integrated Biology/Plant Pathology, 8092, Zurich, Switzerland
    • Kellerhals, Markus. Agroscope Changins-Wadenswil Research Station ACW, Schloss, P.O. Box 185, 8820, Wadenswil, Switzerland
    • Dorn, Silvia. ETH Zurich, Institute of Plant Sciences/Applied Entomology, 8092, Zurich, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:317000
    Summary
    The aim of this study was to assess the genetic basis of rust mite (Aculus schlechtendali) resistance in apple (Malus × domestica). A. schlechtendali infestation of apple trees has increased as a consequence of reduced side effects of modern fungicides on rust mites. An analysis of quantitative trait loci (QTLs) was carried out using linkage map data available for F1 progeny plants of the cultivars ‘Fiesta' × ‘Discovery'. Apple trees representing 160 different genotypes were surveyed for rust mite infestation, each at three different sites in two consecutive years. The distribution of rust mites on the individual apple genotypes was aggregated and significantly affected by apple genotype and site. We identified two QTLs for A. schlechtendali resistance on linkage group 7 of ‘Fiesta'. The AFLP marker E35M42-0146 (20.2cM) and the RAPD marker AE10-400 (45.8cM) were closest positioned to the QTLs and explained between 11.0% and 16.6% of the phenotypic variability. Additionally, putative QTLs on the ‘Discovery' chromosomes 4, 5 and 8 were detected. The SSR marker Hi03a10 identified to be associated to one of the QTLs (AFLP marker E35M42-0146) was traced back in the ‘Fiesta' pedigree to the apple cultivar ‘Wagener'. This marker may facilitate the breeding of resistant apple cultivars by marker assisted selection. Furthermore, the genetic background of rust mite resistance in existing cultivars can be evaluated by testing them for the identified SSR marker