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Sequence diversity and molecular evolution of the merozoite surface antigen 2 of plasmodium falciparum

Felger, Ingrid ; Marshal, Vikki ; Reeder, John ; Hunt, John ; Mgone, Charles ; Beck, Hans-Peter

In: Journal of Molecular Evolution, 1997, vol. 45, no. 2, p. 154-160

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    Titre
    • Sequence diversity and molecular evolution of the merozoite surface antigen 2 of plasmodium falciparum
    Auteur
    • Felger, Ingrid. Institut für Zellbiologie, Universität Witten-Herdecke, Stockumer Str. 10, 58448, Witten, Germany
    • Marshal, Vikki. The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, 3050, Melbourne, Victoria, Australia
    • Reeder, John. The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, 3050, Melbourne, Victoria, Australia
    • Hunt, John. Department of Genetics, University of Hawaii, 1960 East West Road, 96822, Honolulu, HI, USA
    • Mgone, Charles. PNG Institute of Medical Research, P.O. Box 60, Goroka, Papua New Guinea
    • Beck, Hans-Peter. Swiss Tropical Institute, Socinstrasse 57, CH-4002, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Journal of Molecular Evolution, 1997, vol. 45, no. 2, p. 154-160. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:316796
    Summary
    Eleven new alleles of the Plasmodium falciparum merozoite surface antigen 2 (MSA2) from Papua New Guinea were analyzed by direct sequencing of polymerase chain reaction (PCR) products. We have used the sequence information to trace the molecular evolution of MSA2. The repeats of ten alleles belonging to the 3D7 allelic family differed considerably in size, nucleotide sequence, and repeat copy number. In the repeat region of these new alleles, codon usage was extremely biased with an exclusive use of NNT codons. Another new allele sequenced belonged to the FC27 family and confirmed the family-specific conserved structure of 96 and 36 bp repeats. In order to assess sequence microheterogeneity within samples defined as the same genotype by restriction fragment length polymorphism (RFLP), we have analyzed single-strand conformation polymorphism (SSCP) of different samples of the most frequent allele (D10 of the FC27 family) in the study population. No sequence heterogeneity could be detected within the repeat region. Based on analysis of the repeat regions in both allelic families, we discuss the hypothesis of a different evolutionary strategy being represented by each of the allelic families