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Iron acquisition with the natural siderophore enantiomers pyochelin and enantio-pyochelin in Pseudomonas species

Youard, Zeb ; Wenner, Nicolas ; Reimmann, Cornelia

In: BioMetals, 2011, vol. 24, no. 3, p. 513-522

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    Titel
    • Iron acquisition with the natural siderophore enantiomers pyochelin and enantio-pyochelin in Pseudomonas species
    Autor
    • Youard, Zeb. Département de Microbiologie Fondamentale, Université de Lausanne, Bâtiment Biophore, 1015, Lausanne, Switzerland
    • Wenner, Nicolas. Département de Microbiologie Fondamentale, Université de Lausanne, Bâtiment Biophore, 1015, Lausanne, Switzerland
    • Reimmann, Cornelia. Département de Microbiologie Fondamentale, Université de Lausanne, Bâtiment Biophore, 1015, Lausanne, Switzerland
    Dokumententyp
    • Postprint
    OAI-PMH-ID
    • oai:doc.rero.ch:316430
    Summary
    The bacterial siderophore pyochelin is composed of salicylate and two cysteine-derived heterocycles, the second of which is modified by reduction and N-methylation during biosynthesis. In Pseudomonas aeruginosa, the first cysteine residue is converted to its D-isoform during thiazoline ring formation, whereas the second cysteine remains in its L-configuration. Stereochemistry is opposite in the Pseudomonas fluorescens siderophore enantio-pyochelin, in which the first ring originates from L-cysteine and the second ring from D-cysteine. Both siderophores promote growth of the producer organism during iron limitation and induce the expression of their biosynthesis genes by activating the transcriptional AraC-type regulator PchR. However, neither siderophore is functional as an iron carrier or as a transcriptional inducer in the other species, demonstrating that both processes are highly stereospecific. Stereospecificity of pyochelin/enantio-pyochelin-mediated iron uptake is ensured at two levels: (i) by the outer membrane siderophore receptors and (ii) by the cytosolic PchR regulators