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Magnetic-based purification system with simultaneous sample washing and concentration

Ramadan, Qasem ; Lau, Ting ; Ho, Shihan

In: Analytical and Bioanalytical Chemistry, 2010, vol. 396, no. 2, p. 707-714

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    Titre
    • Magnetic-based purification system with simultaneous sample washing and concentration
    Auteur
    • Ramadan, Qasem. Bioelectronics Program, Institute of Microelectronics, 11 Science Park Road, 117685, Singapore, Republic of Singapore
    • Lau, Ting. Nanyang Technological University, Nanyang Avenue, 639798, Singapore, Republic of Singapore
    • Ho, Shihan. Department of Biomedical Engineering, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Republic of Singapore
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2010, vol. 396, no. 2, p. 707-714. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:316370
    Summary
    Simultaneous washing and concentration of magnetic microparticles was demonstrated using a rotational magnetic system under a continuous-flow condition. The rotation of periodically arranged permanent magnets close to a fluidic channel carrying a suspension of magnetic particles allows the trapping and releasing of particles along the fluidic channel in a periodic manner. Each trapping and releasing event resembles one washing cycle in conventional biological assays. Concentration efficiencies of 99.75 ± 0.083% at a flow rate of 200µl/min and 88.10 ± 3.17% at a flow rate of 1,000µl/min and a purification efficiency of 99.10 ± 4.3% at a flow rate of 900µl/min were achieved