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Genome sequence of the temperate bacteriophage PH10 from Streptococcus oralis

van der Ploeg, Jan

In: Virus Genes, 2010, vol. 41, no. 3, p. 450-458

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    Titel
    • Genome sequence of the temperate bacteriophage PH10 from Streptococcus oralis
    Autor
    • van der Ploeg, Jan. Institute of Oral Biology, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Virus Genes, 2010, vol. 41, no. 3, p. 450-458. Springer US; http://www.springer-ny.com
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:315057
    Summary
    Exponential growing cultures of Streptococcus oralis strain OMZ 1038, isolated from human supragingival dental plaque, were found to release a bacteriophage (designated PH10) upon treatment with mitomycin C. The complete genome sequence of phage PH10 was determined. The genome was 31276bp in size and contained 54 open reading frames. The module encoding structural proteins was highly similar to that of Streptococcus pneumoniae prophage PhiSpn_3. The most abundant phage structural protein was encoded by ORF35 and was likely processed by proteolytic cleavage. The putative endolysin from PH10, which contained a muramidase domain and a choline-binding domain, was purified and shown to have lytic activity with S. oralis, S. pneumoniae and Streptococcus mitis, but not with other streptococcal species