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Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities

Han, Jae-Taek ; Bang, Myun-Ho ; Chun, Ock-Kyoung ; Kim, Dae-Ok ; Lee, Chang-Yong ; Baek, Nam-In

In: Archives of Pharmacal Research, 2004, vol. 27, no. 4, p. 390-395

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    Title
    • Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities
    Author
    • Han, Jae-Taek. Eromlife R&D Center, Eromlife Ltd. Co., 135-010, Seoul, Korea
    • Bang, Myun-Ho. Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, 449-701, Suwon, Korea
    • Chun, Ock-Kyoung. Seoul Health & Environmental Research Institute, 138-701, Seoul, Korea
    • Kim, Dae-Ok. Department of Food Science and Technology, Cornell University, 14456, Geneva, New York, USA
    • Lee, Chang-Yong. Department of Food Science and Technology, Cornell University, 14456, Geneva, New York, USA
    • Baek, Nam-In. Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, 449-701, Suwon, Korea
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Archives of Pharmacal Research, 2004, vol. 27, no. 4, p. 390-395. Pharmaceutical Society of Korea
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:314290
    Summary
    The methanol extract obtained from the aerial parts ofAceriphyllum rossii (Saxifragaceae) was fractionated into ethyl acetate (EtOAc),n-BuOH and H2O layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc andn-BuOH layers afforded six flavonol glycosides. They were identified as kaempferol 3-O-β-D-glucopyranoside (astragalin,1), quercetin 3-O-β-D-glucopyranoside (isoquercitrin,2), kaempferol 3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (3), quercetin 3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyrano-side (rutin,4), kaempferol 3-O-[α-L-rhamnopyranosyl (1→4)-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside] (5) and quercetin 3-O-[α-L-rhamnopyranosyl (1→4)-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside] (6) on the basis of several spectral data. The antioxidant activity of the six compounds was investigated using two free radicals such as the ABTS free radical and superoxide anion radical. Compound1 exhibited the highest antioxidant activity in the ABTS2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging method. 100 mg/L of compound1 was equivalent to 72.1±1.4 mg/L of vitamin C, and those of compounds3 and5 were equivalent to 62.7±0.5 mg/L and 54.3±1.3 mg/L of vitamin C, respectively. And in the superoxide anion radical scavenging method, compound5 exhibited the highest activity with an IC50 value of 17.6 ± 0.3 μM. In addition, some physical and spectral data of the flavonoids were confirmed