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New polymorphic microsatellite markers of the endangered meadow viper ( Vipera ursinii ) identified by 454 high-throughput sequencing: when innovation meets conservation

Metzger, César ; Ferchaud, Anne-Laure ; Geiser, Céline ; Ursenbacher, Sylvain

In: Conservation Genetics Resources, 2011, vol. 3, no. 3, p. 589-592

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    Title
    • New polymorphic microsatellite markers of the endangered meadow viper ( Vipera ursinii ) identified by 454 high-throughput sequencing: when innovation meets conservation
    Author
    • Metzger, César. Department of Environmental Sciences, Section of Conservation Biology, University of Basel, St-Johanns-Vorstadt 10, 4056, Basel, Switzerland
    • Ferchaud, Anne-Laure. Laboratoire Biogéographie et Ecologie des Vertébrés, Ecole Pratique de Hautes Etudes, Centre d'Ecologie Fonctionnelle et Evolutive CNRS, 1919 Route de Mende, 34293, Montpellier Cedex 5, France
    • Geiser, Céline. Department of Environmental Sciences, Section of Conservation Biology, University of Basel, St-Johanns-Vorstadt 10, 4056, Basel, Switzerland
    • Ursenbacher, Sylvain. Department of Environmental Sciences, Section of Conservation Biology, University of Basel, St-Johanns-Vorstadt 10, 4056, Basel, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:313743
    Summary
    The Next Generation Sequencing (pyrosequencing) technique allows rapid, low-cost development of microsatellite markers. We have used this technology to develop 14 polymorphic loci for the endangered meadow viper (Vipera ursinii). Based on 37,000 reads, we developed primers for 66 microsatellite loci and found that 14 were polymorphic. The number of alleles per locus varies from 1 to 12 (for 30 individuals tested). At a cost of about 1/3 that of a normal microsatellite development, we were able to define enough microsatellite markers to conduct population genetic studies on a non-model species