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The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions

Monastyrskaya, K. ; Babiychuk, E. ; Schittny, J. ; Rescher, U. ; Gerke, V. ; Mannherz, H.-G ; Draeger, A.

In: Cellular and Molecular Life Sciences CMLS, 2003, vol. 60, no. 12, p. 2702-2709

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    Title
    • The expression levels of three raft-associated molecules in cultivated vascular cells are dependent on culture conditions
    Author
    • Monastyrskaya, K.. Department of Cell Biology, Institute of Anatomy, University of Bern, Bühlstr. 26, 3012, Bern, Switzerland
    • Babiychuk, E.. Department of Cell Biology, Institute of Anatomy, University of Bern, Bühlstr. 26, 3012, Bern, Switzerland
    • Schittny, J.. Department of Cell Biology, Institute of Anatomy, University of Bern, Bühlstr. 26, 3012, Bern, Switzerland
    • Rescher, U.. Institute for Medical Biochemistry, ZMBE, University of Münster, Münster, Germany
    • Gerke, V.. Institute for Medical Biochemistry, ZMBE, University of Münster, Münster, Germany
    • Mannherz, H.-G. Institute of Anatomy and Embryology, Ruhr University, Bochum, Germany
    • Draeger, A.. Department of Cell Biology, Institute of Anatomy, University of Bern, Bühlstr. 26, 3012, Bern, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Cellular and Molecular Life Sciences CMLS, 2003, vol. 60, no. 12, p. 2702-2709. Birkhäuser-Verlag
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:312429
    Summary
    Relaying a signal across the plasma membrane requires functional connections between the partner molecules. Membrane microdomains or lipid rafts provide an environment in which such specific interactions can take place. The integrity of these sites is often taken for granted when signalling pathways are investigated in cell culture. However, it is well known that smooth muscle and endothelial cells undergo cytoskeletal rearrangements during monolayer culturing. Likewise affected - and with potentially important consequences for signalling events - is the organization of the plasma membrane. The expression levels of three raft markers were massively upregulated, and raft-associated 5′-nucleotidase activity increased in conventional monolayer cultures as compared with a spheroidal coculture model, shown to promote the differentiation of endothelial cells. Our data point to a shift of raft components in monolayer cultures and demonstrate potential advantages of the spheroid coculture system for investigation of raft-mediated signalling events in endothelial cells