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Non-Invasive Time-Lapsed Monitoring and Quantification of Engineered Bone-Like Tissue

Hagenmüller, Henri ; Hofmann, Sandra ; Kohler, Thomas ; Merkle, Hans ; Kaplan, David ; Vunjak-Novakovic, Gordana ; Müller, Ralph ; Meinel, Lorenz

In: Annals of Biomedical Engineering, 2007, vol. 35, no. 10, p. 1657-1667

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    Titre
    • Non-Invasive Time-Lapsed Monitoring and Quantification of Engineered Bone-Like Tissue
    Auteur
    • Hagenmüller, Henri. Institute for Biomechanics, ETH Zurich, 8093, Zurich, Switzerland
    • Hofmann, Sandra. Institute of Pharmaceutical Sciences, ETH Zurich, 8093, Zurich, Switzerland
    • Kohler, Thomas. Institute for Biomechanics, ETH Zurich, 8093, Zurich, Switzerland
    • Merkle, Hans. Institute of Pharmaceutical Sciences, ETH Zurich, 8093, Zurich, Switzerland
    • Kaplan, David. Department of Biomedical Engineering, Tufts University, 02155, Medford, MA, USA
    • Vunjak-Novakovic, Gordana. Department of Biomedical Engineering, Columbia University, 10027, New York, NY, USA
    • Müller, Ralph. Institute for Biomechanics, ETH Zurich, 8093, Zurich, Switzerland
    • Meinel, Lorenz. Institute of Pharmaceutical Sciences, ETH Zurich, 8093, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Annals of Biomedical Engineering, 2007, vol. 35, no. 10, p. 1657-1667. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:311891
    Summary
    The formation of bone-like tissue from human mesenchymal stem cells (hMSC) cultured in osteogenic medium on silk fibroin scaffolds was monitored and quantified over 44days in culture using non-invasive time-lapsed micro-computed tomography (μCT). Each construct was imaged nine times insitu. From μCT imaging, detailed morphometrical data on bone volume density, surface-to-volume ratio, trabecular thickness, trabecular spacing, and the structure model index and tissue mineral density were obtained. μCT irradiation did not impact the osteogenic performance of hMSCs based on DNA content, alkaline phosphatase activity, and calcium deposition when compared to non-exposed control samples. Bone-like tissue formation initiated at day 10 of the culture with the deposition of small mineralized clusters. Tissue mineral density increased linearly over time. The surface-to-volume ratio of the bone-like tissues converged asymptotically to 26mm−1. Although in vitro formation of bone-like tissue started from clusters, the overall bone volume was not predictable from the time, number, and size of initially formed bone-like clusters. Based on microstructural analysis, the morphometry of the tissue-engineered constructs was found to be in the range of human trabecular bone. In future studies, non-invasive, time-lapsed monitoring may enable researchers to culture tissues in vitro, right until the development of a desired morphology is accomplished. Our data demonstrate the feasibility of qualitatively and quantitatively detailing the spatial and temporal mineralization of bone-like tissue formation in tissue engineering