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Dose-response effect of interleukin (IL)-1β, tumour necrosis factor (TNF)-α, and interferon-γ on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells

Bersinger, Nick ; Günthert, Andreas ; McKinnon, Brett ; Johann, Silke ; Mueller, Michael

In: Archives of Gynecology and Obstetrics, 2011, vol. 283, no. 6, p. 1291-1296

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    Titel
    • Dose-response effect of interleukin (IL)-1β, tumour necrosis factor (TNF)-α, and interferon-γ on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells
    Autor
    • Bersinger, Nick. Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland
    • Günthert, Andreas. Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland
    • McKinnon, Brett. Department of Clinical Research, University of Berne, Berne, Switzerland
    • Johann, Silke. Hôpital du jura bernois, St-Imier, Switzerland
    • Mueller, Michael. Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Archives of Gynecology and Obstetrics, 2011, vol. 283, no. 6, p. 1291-1296. Springer-Verlag
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:311420
    Summary
    Purpose: The production of epithelial neutrophil activating peptide-78 (NA-78) and the interleukins IL-8 and IL-6 by endometrial stromal cells is stimulated by pro-inflammatory interleukin-1 (IL-1) and tumour necrosis factor-α (TNF-α). IL-8 is suggested to play a role in the pathogenesis of endometriosis, and in these women the peritoneal fluid concentrations of ENA-78 and IL-8 are increased. TNF-α has been tested together with interferon-γ because of their cooperative stimulation of IL-6. The release of IL-8, however, is inhibited with increasing interferon levels. The aim of the study was the analysis of the production of ENA-78, IL-6 and IL-8 by cultured human endometrial stromal cells in the presence of varying concentrations of IL-1β, TNF-α, and interferon-γ. Methods: Eutopic endometrial tissue was obtained from seven cycling, endometriosis-free women undergoing laparoscopy for reasons of infertility or pain. The release of ENA-78, IL-8 and IL-6 by the isolated and monolayer cultured stromal cell fraction in the presence of IL-1β (0.08 to 50ng/mL), TNF-α, and interferon-γ (both 20 to 500ng/mL) was determined. Results: IL-1β stimulated the production of IL-8, IL-6, and ENA-78 dose dependently from 0.08 to 2.0ng/mL (ENA-78) or to 10ng/mL (IL-8, IL-6); at 50ng/mL a decrease in release was observed for IL-8 and IL-6. TNF-α stimulation yielded a plateau between 20 and 100ng/mL. Interferon-γ stimulated IL-6 and inhibited IL-8 production above 20ng/mL. ENA-78 release was largely unaffected by interferon-γ. Conclusions: IL-1β and TNF-α stimulate stromal cytokine production cumulatively with different dose-response curves. The presence of interferon-γ has opposite effects on IL-8 and IL-6. TNF-α and interferon-γ should be investigated separately in future in vitro studies with endometrial cells and explants