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Functional expression of arginine kinase improves recovery from pH stress of Escherichia coli

Canonaco, Fabrizio ; Schlattner, Uwe ; Wallimann, Theo ; Sauer, Uwe

In: Biotechnology Letters, 2003, vol. 25, no. 13, p. 1013-1017

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    Titre
    • Functional expression of arginine kinase improves recovery from pH stress of Escherichia coli
    Auteur
    • Canonaco, Fabrizio. Institute of Biotechnology, ETH Zürich, CH-8093, Zürich, Switzerland
    • Schlattner, Uwe. Institute of Biotechnology, ETH Zürich, CH-8093, Zürich, Switzerland
    • Wallimann, Theo. Institute of Biotechnology, ETH Zürich, CH-8093, Zürich, Switzerland
    • Sauer, Uwe. Institute of Biotechnology, ETH Zürich, CH-8093, Zürich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Biotechnology Letters, 2003, vol. 25, no. 13, p. 1013-1017. Kluwer Academic Publishers
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:311182
    Summary
    Acid stress in Escherichia coli involves a complex resource- and energy-consuming response mechanism. By overexpression of arginine kinase from Limulus polyphemus in E.coli, we improved the recovery from a transient pH stress. While wild type E.coli resumed growth after a transient pH reduction to pH3 for 1h with a rate that was 25% lower than before the stress, the arginine kinase expressing strain continued to grow as rapidly as before. This effect is presumably caused by the physiological function of arginine kinase as a short term energy buffer in the form of phosphoarginine, but a pH-buffering effect cannot be excluded