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Cardiovascular effects of fentanyl in conscious rats

Baechtold, François ; Cavadas, Claudia ; Gasser, Didier ; Markert, Michèle ; Grouzmann, Eric ; Peterson, Kirk ; Waeber, Bernard ; Feihl, François

In: Pflügers Archiv, 2001, vol. 443, no. 1, p. 155-162

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    Titel
    • Cardiovascular effects of fentanyl in conscious rats
    Autor
    • Baechtold, François. Division of Clinical Pathophysiology, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Cavadas, Claudia. Division of Hypertension, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Gasser, Didier. Division of Clinical Pathophysiology, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Markert, Michèle. Laboratory of Clinical Chemistry, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Grouzmann, Eric. Division of Hypertension, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Peterson, Kirk. Division of Cardiology, Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA, USA
    • Waeber, Bernard. Division of Clinical Pathophysiology, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    • Feihl, François. Division of Clinical Pathophysiology, University Hospital, 1011 Lausanne, Switzerland, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Pflügers Archiv, 2001, vol. 443, no. 1, p. 155-162. Springer-Verlag
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:310997
    Summary
    Abstract.: The polymicrobial sepsis induced by cecal ligation and puncture (CLP) in the rat is widely used in shock research. For ethical reasons, narcotic analgesics are often administered in this model, with the potential risk of confounding effects. In conscious non-septic rats, we investigated the cardiovascular effects of a continuous i.v. infusion of fentanyl (20µg/kg per h) administered with fluid loading (10ml/kg per h) for 24h, a regimen commonly applied in rat CLP. Animals were randomly allocated to receive analgesia with fluid loading (Fentanyl group), or fluid loading alone (Control). All endpoints were assessed after 24h of infusion. At that time, Control animals had mild respiratory alkalosis, which was essentially abolished by fentanyl. Analgesia mildly elevated the plasma norepinephrine levels [median (interquartile range): Control 232pg/ml (0-292), Fentanyl 302pg/ml (234-676), P=0.045] but was devoid of any effect on blood pressure, heart rate, cardiac output (mean ±SD: Control 388±61ml/kg per min, Fentanyl 382±62ml/kg per min, P=0.87) and indices of left ventricular function derived from high-fidelity recordings of left ventricular pressure (dP/dt max: Control 11782±2324mmHg/s, Fentanyl 12107±2816mmHg/s, P=0.77). In ex vivo experiments carried out immediately after animal sacrifice, no differences were noted between the Control and Fentanyl groups in the sensitivity of endothelium-intact aortic rings to norepinephrine-induced vasoconstriction (-logEC50: Control 8.78±0.28, Fentanyl 8.83±0.26, P=0.52) or acetylcholine-induced vasodilatation (-logEC50: Control 7.00±0.37, Fentanyl 7.06±0.26±0.53, P=0.75). In conclusion, the present data provide no contraindication, and even some support for the ethical use of a high dose i.v. infusion of fentanyl in cardiovascular studies of conscious catheterized rats undergoing CLP or other painful procedures