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Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures

Yildirim, Selcuk ; Konrad, Daniel ; Calvez, Ségolène ; Drider, Djamel ; Prévost, Hervé ; Lacroix, Christophe

In: Applied Microbiology and Biotechnology, 2007, vol. 77, no. 3, p. 525-531

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    Title
    • Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures
    Author
    • Yildirim, Selcuk. Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH (Swiss Federal Institute of Technology) Zurich, LFV C20 Schmelzbergstrasse 7, CH-8092, Zurich, Switzerland
    • Konrad, Daniel. Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH (Swiss Federal Institute of Technology) Zurich, LFV C20 Schmelzbergstrasse 7, CH-8092, Zurich, Switzerland
    • Calvez, Ségolène. UMR-INRA 1014 SECALIM, ENTIAA, BP 82225, 44322, Nantes Cedex 3, France
    • Drider, Djamel. UMR-INRA 1014 SECALIM, ENTIAA, BP 82225, 44322, Nantes Cedex 3, France
    • Prévost, Hervé. UMR-INRA 1014 SECALIM, ENTIAA, BP 82225, 44322, Nantes Cedex 3, France
    • Lacroix, Christophe. Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH (Swiss Federal Institute of Technology) Zurich, LFV C20 Schmelzbergstrasse 7, CH-8092, Zurich, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Applied Microbiology and Biotechnology, 2007, vol. 77, no. 3, p. 525-531. Springer-Verlag
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:309929
    Summary
    To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0-2mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06g cdw/l and 18 ± 1mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6g cdw/l, respectively) and bacteriocin yields (30 and 74mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks