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Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper

Slaveykova, Vera ; Guignard, Cédric ; Eybe, Tanja ; Migeon, Henri-Noël ; Hoffmann, Lucien

In: Analytical and Bioanalytical Chemistry, 2009, vol. 393, no. 2, p. 583-589

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    Titre
    • Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper
    Auteur
    • Slaveykova, Vera. Environmental Biophysical Chemistry, Environmental Science and Technology Institute, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 2, 1015, Lausanne, Switzerland
    • Guignard, Cédric. Department of Environment and Agro-Biotechnologies (EVA), Public Research Center—Gabriel Lippmann, 41 rue du Brill, 4422, Belvaux, Luxembourg
    • Eybe, Tanja. Department of Environment and Agro-Biotechnologies (EVA), Public Research Center—Gabriel Lippmann, 41 rue du Brill, 4422, Belvaux, Luxembourg
    • Migeon, Henri-Noël. Department of Science and Analysis of Materials (SAM), Public Research Center—Gabriel Lippmann, 41 rue du Brill, 4422, Belvaux, Luxembourg
    • Hoffmann, Lucien. Department of Environment and Agro-Biotechnologies (EVA), Public Research Center—Gabriel Lippmann, 41 rue du Brill, 4422, Belvaux, Luxembourg
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2009, vol. 393, no. 2, p. 583-589. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:309801
    Summary
    This work demonstrates the capabilities of nanoscale secondary-ion mass spectrometry, using the Cameca NanoSIMS50 ion microprobe, to detect and image the copper-ion distribution in microalgal cells exposed to nanomolar and micromolar copper concentrations. In parallel to 63Cu− secondary-ion maps, images of 12C−, 12C14N−, and 31P− secondary ions were collected and analysed. A correlation of 63Cu− secondary-ion maps with those found for 12C14N− and 31P− demonstrated the possible association of Cu with cell components rich in proteins and phosphorus. The results highlighted the potential of NanoSIMS for intracellular tracking of essential trace elements such as Cu in single cells of the microalga Chlorella kesslerii. Figure 12C14N-, 63Cu- secondary-ion distributions in algal cell