Fulltext

Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs

Vanderschuren, Hervé ; Akbergenov, Rashid ; Pooggin, Mikhail ; Hohn, Thomas ; Gruissem, Wilhelm ; Zhang, Peng

In: Plant Molecular Biology, 2007, vol. 64, no. 5, p. 549-557

Ajouter à la liste personnelle
    Titre
    • Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs
    Auteur
    • Vanderschuren, Hervé. Institute of Plant Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zürich, Switzerland
    • Akbergenov, Rashid. Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056, Basel, Switzerland
    • Pooggin, Mikhail. Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056, Basel, Switzerland
    • Hohn, Thomas. Institute of Botany, University of Basel, Schönbeinstrasse 6, 4056, Basel, Switzerland
    • Gruissem, Wilhelm. Institute of Plant Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zürich, Switzerland
    • Zhang, Peng. Institute of Plant Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zürich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Plant Molecular Biology, 2007, vol. 64, no. 5, p. 549-557. Kluwer Academic Publishers
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:309634
    Summary
    Expression of double-stranded RNA (dsRNA) homologous to virus sequences can effectively interfere with RNA virus infection in plant cells by triggering RNA silencing. Here we applied this approach against a DNA virus, African cassava mosaic virus (ACMV), in its natural host cassava. Transgenic cassava plants were developed to express small interfering RNAs (siRNA) from a CaMV 35S promoter-controlled, intron-containing dsRNA cognate to the common region-containing bidirectional promoter of ACMV DNA-A. In two of three independent transgenic lines, accelerated plant recovery from ACMV-NOg infection was observed, which correlates with the presence of transgene-derived siRNAs 21-24nt in length. Overall, cassava mosaic disease symptoms were dramatically attenuated in these two lines and less viral DNA accumulation was detected in their leaves than in those of wild-type plants. In a transient replication assay using leaf disks from the two transgenic lines, strongly reduced accumulation of viral single-stranded DNA was observed. Our study suggests that a natural RNA silencing mechanism targeting DNA viruses through production of virus-derived siRNAs is turned on earlier and more efficiently in transgenic plants expressing dsRNA cognate to the viral promoter and common region