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Toxicity in Neuronal Cells Caused by Cerebrospinal Fluid from Pneumococcaland Gram-Negative Meningitis

Täuber, Martin G. ; Sachdeva, Meena ; Kennedy, Stephen L. ; Loetscher, Hansruedi ; Lesslauer, Werner

In: Journal of Infectious Diseases, 1992, vol. 166, no. 5, p. 1045-1050

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    Titre
    • Toxicity in Neuronal Cells Caused by Cerebrospinal Fluid from Pneumococcaland Gram-Negative Meningitis
    Auteur
    • Täuber, Martin G.. Microbial Pathogenesis Unit, San Francisco General Hospital, and Department of Medicine, University of California, San Francisco, Basel, Switzerland
    • Sachdeva, Meena. Microbial Pathogenesis Unit, San Francisco General Hospital, and Department of Medicine, University of California, San Francisco, Basel, Switzerland
    • Kennedy, Stephen L.. Microbial Pathogenesis Unit, San Francisco General Hospital, and Department of Medicine, University of California, San Francisco, Basel, Switzerland
    • Loetscher, Hansruedi. Microbial Pathogenesis Unit, San Francisco General Hospital, and Department of Medicine, University of California, San Francisco, Basel, Switzerland
    • Lesslauer, Werner. Microbial Pathogenesis Unit, San Francisco General Hospital, and Department of Medicine, University of California, San Francisco, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Infectious Diseases, 1992, vol. 166, no. 5, p. 1045-1050. The University of Chicago Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:304034
    Summary
    To identify neurotoxic factors in meningitis, a neuronal cell line (HN33.1) was exposed to cerebrospinal fluid (CSF) obtained from rabbits with pneumococcal meningitis or Escherichia coli meningitis or 2 h and 6 h after meningitis was induced by proinflammatory bacterial products (pneumococcal cell walls, endotoxin). CSF from all types of meningitis induced similar degrees of cytotoxicity. When a soluble tumor necrosis factor (TNF) receptor that completely blocked TNF-mediated toxicity at 10-7 M was used, all toxicity in meningitis caused by E. coli, endotoxin, or pneumococcal cell wall administration (2 h afterwards) was mediated by TNF. In contrast, CSF from animals with meningitis caused by live pneumococci or pneumococcal cell wall injection (6 h afterwards) retained cytotoxicity in the presence of the TNF receptor. Thus, in established pneumococcal meningitis, but not in the other forms of meningitis, TNF is not the only component toxic in this neuronal cell line