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Cytokines and Inflammatory Mediators [30-39] : 30. The LPS Stimulated Production of Interleukin-10 is not Associated with -819C/T and -592C/A Promoter Polymorphisms in Healthy Indian Subjects

Shukla, Skanda ; Lawrence, Able ; Aggarwal, Amita ; Naik, Sita ; Gullick, Nicola J. ; Evans, Hayley G. ; Jayaraj, David ; Kirkham, Bruce W. ; Taams, Leonie S. ; Judah, Sunita M. ; Nixon, Nicola ; Dawes, Peter ; Mattey, Derek L. ; Yeo, Lorraine ; Schmutz, Caroline ; Toellner, Kai-Michael ; Salmon, Mike ; Filer, Andrew D. ; Buckley, Christopher ; Raza, Karim ; Scheel-Toellner, Dagmar ; Hashizume, M. ; Yoshida, H. ; Koike, N. ; Suzuki, M. ; Mihara, M. ; Stavropoulos-Kalinoglou, Antonios ; Metsios, Giorgos S. ; Douglas, Karen M. ; Panoulas, Vasileios F. ; Koutedakis, Yiannis ; Kitas, George D. ; Church, Leigh D. ; Filer, Andrew D. ; Hildago, Ester ; Howlett, Katherine ; Thomas, Andrew ; Rapecki, Stephen ; Scheel-Toellner, Dagmar ; Buckley, Christopher D. ; Raza, Karim ; Juarez, Maria ; Kolasinski, James ; Govindan, Jyothish ; Quilter, Annette ; Williamson, Lyn ; Collins, David A. ; Price, Elizabeth J. ; Gasparyan, Armen Y. ; Stavropoulos-Kalinoglou, Antonios ; Toms, Tracey E. ; Douglas, Karen ; Kitas, George D. ; Lachmann, H. J. ; Kuemmerle-Deschner, J. B. ; Hachulla, E. ; Hoyer, J. ; Smith, J. ; Leslie, K. ; Kone-Paut, I. ; Braun, J. ; Widmer, A. ; Patel, N. ; Preiss, R. ; Hawkins, P. N.

In: Rheumatology, 2010, vol. 49, p. i45-i48

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    Titre
    • Cytokines and Inflammatory Mediators [30-39]
      30. The LPS Stimulated Production of Interleukin-10 is not Associated with -819C/T and -592C/A Promoter Polymorphisms in Healthy Indian Subjects
    Auteur
    • Shukla, Skanda. Immunology, SGPGIMS, Lucknow, India
    • Lawrence, Able. Immunology, SGPGIMS, Lucknow, India
    • Aggarwal, Amita. Immunology, SGPGIMS, Lucknow, India
    • Naik, Sita. Immunology, SGPGIMS, Lucknow, India
    • Gullick, Nicola J.. CMCBI, King's College, London, UK; NIHR Comprehensive Biomedical Research Centre, Guy's and St Thomas' NHS Foundation Trust and King's College, London, UK; Rheumatology, Guy's and St Thomas' NHS Foundation Trust, London, UK
    • Evans, Hayley G.. CMCBI, King's College, London, UK; NIHR Comprehensive Biomedical Research Centre, Guy's and St Thomas' NHS Foundation Trust and King's College, London, UK; Rheumatology, Guy's and St Thomas' NHS Foundation Trust, London, UK
    • Jayaraj, David. CMCBI, King's College, London, UK; NIHR Comprehensive Biomedical Research Centre, Guy's and St Thomas' NHS Foundation Trust and King's College, London, UK; Rheumatology, Guy's and St Thomas' NHS Foundation Trust, London, UK
    • Kirkham, Bruce W.. CMCBI, King's College, London, UK; NIHR Comprehensive Biomedical Research Centre, Guy's and St Thomas' NHS Foundation Trust and King's College, London, UK; Rheumatology, Guy's and St Thomas' NHS Foundation Trust, London, UK
    • Taams, Leonie S.. CMCBI, King's College, London, UK; NIHR Comprehensive Biomedical Research Centre, Guy's and St Thomas' NHS Foundation Trust and King's College, London, UK; Rheumatology, Guy's and St Thomas' NHS Foundation Trust, London, UK
    • Judah, Sunita M.. Institute for Science and Technology in Medicine, Keele University, Stoke On Trent, UK; Rheumatology, Haywood Hospital, Stoke On Trent, UK
    • Nixon, Nicola. Institute for Science and Technology in Medicine, Keele University, Stoke On Trent, UK; Rheumatology, Haywood Hospital, Stoke On Trent, UK
    • Dawes, Peter. Institute for Science and Technology in Medicine, Keele University, Stoke On Trent, UK; Rheumatology, Haywood Hospital, Stoke On Trent, UK
    • Mattey, Derek L.. Institute for Science and Technology in Medicine, Keele University, Stoke On Trent, UK; Rheumatology, Haywood Hospital, Stoke On Trent, UK
    • Yeo, Lorraine. Immunology, SGPGIMS, Lucknow, India
    • Schmutz, Caroline. Immunology, SGPGIMS, Lucknow, India
    • Toellner, Kai-Michael. Immunology, SGPGIMS, Lucknow, India
    • Salmon, Mike. Immunology, SGPGIMS, Lucknow, India
    • Filer, Andrew D.. Immunology, SGPGIMS, Lucknow, India
    • Buckley, Christopher. Immunology, SGPGIMS, Lucknow, India
    • Raza, Karim. Immunology, SGPGIMS, Lucknow, India
    • Scheel-Toellner, Dagmar. Immunology, SGPGIMS, Lucknow, India
    • Hashizume, M.. Immunology, SGPGIMS, Lucknow, India
    • Yoshida, H.. Immunology, SGPGIMS, Lucknow, India
    • Koike, N.. Immunology, SGPGIMS, Lucknow, India
    • Suzuki, M.. Immunology, SGPGIMS, Lucknow, India
    • Mihara, M.. Immunology, SGPGIMS, Lucknow, India
    • Stavropoulos-Kalinoglou, Antonios. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Metsios, Giorgos S.. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Douglas, Karen M.. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Panoulas, Vasileios F.. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Koutedakis, Yiannis. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Kitas, George D.. Rheumatology, Dudley Group of Hospitals, Dudley, UK; School of Sports and Exercise Science, University of Birmingham, Birmingham, UK; School of Sports, Performing Arts and Leisure, University of Wolverhampton, Walsall, UK
    • Church, Leigh D.. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Filer, Andrew D.. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Hildago, Ester. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Howlett, Katherine. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Thomas, Andrew. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Rapecki, Stephen. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Scheel-Toellner, Dagmar. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Buckley, Christopher D.. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Raza, Karim. Rheumatology Research Group, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK; The Royal Orthopaedic Hospital, Birmingham, UK; UCB-Celltech, Slough, UK
    • Juarez, Maria. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Kolasinski, James. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Govindan, Jyothish. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Quilter, Annette. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Williamson, Lyn. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Collins, David A.. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Price, Elizabeth J.. Rheumatology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Diabetes and Endocrinology, The Great Western Hospital NHS Foundation Trust, Swindon, UK; Cardiology, The Great Western Hospital NHS Foundation Trust, Swindon, UK
    • Gasparyan, Armen Y.. Immunology, SGPGIMS, Lucknow, India
    • Stavropoulos-Kalinoglou, Antonios. Immunology, SGPGIMS, Lucknow, India
    • Toms, Tracey E.. Immunology, SGPGIMS, Lucknow, India
    • Douglas, Karen. Immunology, SGPGIMS, Lucknow, India
    • Kitas, George D.. Immunology, SGPGIMS, Lucknow, India
    • Lachmann, H. J.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Kuemmerle-Deschner, J. B.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Hachulla, E.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Hoyer, J.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Smith, J.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Leslie, K.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Kone-Paut, I.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Braun, J.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Widmer, A.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Patel, N.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Preiss, R.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    • Hawkins, P. N.. Royal Free and University College Medical School, London, UK; Universitätsklinikum Tübingen, Klinik fuer Kinder-und Jugendmedizin, Germany; Hopital Claude Huriez, Lille Cedex, France; Univ.- Klinikum Gieβen und Marburg, Marburg, Germany; University of Wisconsin Hospital and Clinics, Madison, WI; UCSF, School of Medicine, San Francisco, CA, USA; Hopital Kremlin Bicetre, Le Kremlin Bicetre, France; Rheumazentrum Ruhrgebiet, Herne, Germany; Novartis, Basel, Switzerland; Novartis, East Hanover, NJ, USA
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:303406
    Summary
    Background: Interleukin-10 is a pivotal immunoregulatory cytokine with pleiotropic effects on the immune system. IL-10 promoter polymorphisms have been associated with disease susceptibility and the ability to secrete IL-10 in vitro. We suspected that the association of the widely studied -819C/T and -592C/A polymorphisms with the IL-10 production might vary between ethnic groups. Therefore, we examined the association of -819 C/T and -592 C/A promoter polymorphisms with in vitro LPS stimulated secretion of IL-10 in normal healthy Indian volunteers. Methods: Peripheral blood was collected from 103 healthy volunteers and diluted whole blood cultures were set up with 100 ng/ml of LPS as stimulant: supernatant was collected at 24 h and IL-10 levels were assayed by ELISA. Genotyping was done for -819C/T polymorphism in 101 individuals and -592C/A polymorphism in 68 individuals by polymerase chain reaction followed by RFLP. The differences in IL-10 production between the genotypes were analysed by ANOVA. Results: There were 30, 47 and 24 individuals with the CC, CT and TT genotypes with a minor allele (T) frequency of 47% for the -819C/T polymorphism. The CC and TT genotypes at position -819 were strongly associated with CC and AA genotypes at -592 position suggestive of strong linkage disequilibrium. There was no association between the -819 genotype and the in vitro LPS stimulated IL-10 levels. Conclusions: The -819C/T and the -592 C/A polymorphisms of the IL-10 promoter region are not significantly associated with LPS stimulated IL-10 production healthy Indian subjects. Disclosure statement: All authors have declared no conflicts of interest