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Susceptibility testing of Haemophilus influenzae— an international collaborative study in quality assessment

Yeo, S. F. ; Akalln, E. ; Arikan, S. ; Auckenthaler, R. ; Bergan, T. ; Dornbusch, K. ; Howard, A. J. ; Hryniewicz, W. ; Jones, R. N. ; Koupari, G. ; Legakis, N. J. ; McLaughlin, J. ; Ozkuyumcu, C. ; Percival, A. ; Phillips, I. ; Reeves, D. ; Spencer, R. ; Warren, R. E. ; Williams, J. D.

In: Journal of Antimicrobial Chemotherapy, 1996, vol. 38, no. 3, p. 363-386

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    Titre
    • Susceptibility testing of Haemophilus influenzae— an international collaborative study in quality assessment
    Auteur
    • Yeo, S. F.. London Hospital Medical College London, UK
    • Akalln, E.. Haceteppe University Ankara, Turkey
    • Arikan, S.. University Hospital of Geneva Switzerland
    • Auckenthaler, R.. University Hospital of Geneva Switzerland
    • Bergan, T.. University of Oslo Norway
    • Dornbusch, K.. Karolinska Hospital Stockholm, Sweden
    • Howard, A. J.. Bangor Public Health Laboratory Gwynedd, UK
    • Hryniewicz, W.. National Institute of Hygiene Warsaw, Poland
    • Jones, R. N.. University of Iowa Iowa, USA
    • Koupari, G.. University of Athens Medical School Athens, Greece
    • Legakis, N. J.. University of Athens Medical School Athens, Greece
    • McLaughlin, J.. University of New Mexico Medical Center Albuquerque, NM, USA
    • Ozkuyumcu, C.. Haceteppe University Ankara, Turkey
    • Percival, A.. Royal Liverpool University Hospital Liverpool, UK
    • Phillips, I.. United Medical & Dental School London, UK
    • Reeves, D.. Southmead Health Services Bristol, UK
    • Spencer, R.. Public Health Laboratory Bristol, UK
    • Warren, R. E.. Royal Shrewsbury Hospital Shrewsbury, UK
    • Williams, J. D.. London Hospital Medical College London, UK
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 1996, vol. 38, no. 3, p. 363-386. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:303028
    Summary
    In order to compare the prevalence of antibiotic resistance in different geographical areas, it is necessary to ensure that agreement is achieved between laboratories on the assignment of strains to ‘susceptible' and ‘resistant' categories. An international quality assessment study, involving 15 laboratories in eight countries, was performed to investigate the standard of performance of the susceptibility testing of Haemophilus influenzae. One hundred and fifty strains of H. influenzae were distributed from the London Hospital Medical College (LHMC) to all laboratories who were asked to test the susceptibility of the strains to ampicillin, chloramphenicol, tetracycline, trimethoprim, cephalosporins and ciprofloxacin. Laboratories were also asked to provide the details of methodology to test the susceptibility. Significant discrepancy between the LHMC and the participating laboratories appeared in the detection of resistance to ampicillin (especially β-lactamase-negative strains resistant to ampicillin) as well as the assignment of susceptibility and resistance to chloramphenicol, tetracycline and trimethoprim. Often these reflected the use of inappropriate breakpoints which led to erroneous assignment of susceptibility. Other variations including disc content, medium and supplement, inoculum as well as failure to measure zone sizes properly also led to some repeating anomalies