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Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in Bacillus subtilis

Zamboni, Nicola ; Sauer, Uwe

In: FEMS Microbiology Letters, 2003, vol. 226, no. 1, p. 121-126

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    Title
    • Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in Bacillus subtilis
    Author
    • Zamboni, Nicola. Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland
    • Sauer, Uwe. Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:302277
    Summary
    The metabolic impact of electron rerouting in the respiratory chain of Bacillus subtilis was quantitatively assessed during batch growth of quinol oxidase mutants by 13C-tracer experiments. While disruption of the low-coupling cytochrome bd oxidase was without any apparent phenotype, deletion of the high-coupling cytochrome aa3 oxidase caused a severe reduction of tricarboxylic acid cycle fluxes and increased overflow metabolism. Since the product-corrected biomass yields were identical in mutants and parent, the results show that efficient ATP generation is not overly important for exponential growth of B. subtilis in batch culture