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A novel IncQ plasmid type harbouring a class 3 integron from Escherichia coli

Poirel, Laurent ; Carattoli, Alessandra ; Bernabeu, Sandrine ; Bruderer, Thomas ; Frei, Reno ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2010, vol. 65, no. 8, p. 1594-1598

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    Titre
    • A novel IncQ plasmid type harbouring a class 3 integron from Escherichia coli
    Auteur
    • Poirel, Laurent. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France
    • Carattoli, Alessandra. Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita', Rome, Italy
    • Bernabeu, Sandrine. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France
    • Bruderer, Thomas. Department of Microbiology, University Hospital, Basel, Switzerland
    • Frei, Reno. Department of Microbiology, University Hospital, Basel, Switzerland
    • Nordmann, Patrice. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 2010, vol. 65, no. 8, p. 1594-1598. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:302154
    Summary
    Objectives To determine the genetic structures at the origin of the mobilization of the extended-spectrum β-lactamase (ESBL) blaGES-1 gene in an Escherichia coli clinical isolate. Methods ESBL-encoding genes and class 1 or class 3 integron-specific motifs were screened. Conjugation experiments were performed to determine whether the plasmid-carrying blaGES-1 gene was self-transferable. Plasmid sequencing was achieved by a primer-walking approach. Results The blaGES-1 gene was located in a class 3 integron. That unusual genetic structure was itself located on an ∼9 kb plasmid, pQ7, which was not self-transferable. Sequence analysis revealed that plasmid pQ7 belonged to a novel subtype of the IncQ group. Conclusions This study identified for the first time the blaGES-1 gene in E. coli and in Switzerland. It describes a novel IncQ-type plasmid subgroup that possesses original features, in particular iteron sequences that constitute a hot spot for integration of foreign DNA