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Identification of Two Cryptic Species within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics

Mitrovski-Bogdanović, Ana ; Petrović, Andjeljko ; Mitrović, Milana ; Ivanović, Ana ; Žikić, Vladimir ; Starý, Petr ; Vorburger, Christoph ; Tomanović, Željko

In: Annals of the Entomological Society of America, 2013, vol. 106, no. 2, p. 170-180

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    Titre
    • Identification of Two Cryptic Species within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics
    Auteur
    • Mitrovski-Bogdanović, Ana. Institute of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
    • Petrović, Andjeljko. Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
    • Mitrović, Milana. Department of Plant Pests, Institute for Plant Protection and Environment, Banatska 33, 11080 Zemun, Serbia.
    • Ivanović, Ana. Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
    • Žikić, Vladimir. Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Višegradska 33, 18000 Nisš, Serbia.
    • Starý, Petr. Laboratory of Aphidology, Institute of Entomology, Biology Centre, Academy of Sciences of the Czech Republic, Branisšovská 31, 37005 Ĉ eské Budě jovice, Czech Republic.
    • Vorburger, Christoph. Institute of Integrative Biology, ETH Zürich, Switzerland, and EAWAG, Swiss Federal; Institute of Aquatic Science and Technology, Ü berlandstrasse 133, 8600 Dübendorf, Switzerland.
    • Tomanović, Željko. Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Annals of the Entomological Society of America, 2013, vol. 106, no. 2, p. 170-180. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:302020
    Summary
    The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium