Fulltext

Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

op den Camp, Roel G.L. ; Kuhlemeier, Cris

In: Nucleic Acids Research, 1998, vol. 26, no. 9, p. 2058-2062

Aggiungi alla tua lista
    Titre
    • Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination
    Auteur
    • op den Camp, Roel G.L.. Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Berne, Switzerland
    • Kuhlemeier, Cris. Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Berne, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Nucleic Acids Research, 1998, vol. 26, no. 9, p. 2058-2062. Oxford University Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301726
    Summary
    Eukaryotic translation initiation factor eIF-4A is a member of the DEAD box family of RNA helicases and RNA-dependent ATPases. In tobacco, eIF-4A is encoded by a gene family with one isoform, eIF-4A8, being exclusively expressed in pollen. This pollen-specific isoform is a candidate for mediating translational control in the developing gametophyte. Here we show that eIF-4A is barely phosphorylated in mature pollen, but during pollen tube germination, two isoforms of eIF-4A become phosphorylated. Phosphoamino acid analysis indicated phosphorylation of threonine. In order to determine whether pollen-specific eIF-4A8 is among the phosphorylated isoforms, we raised transgenic tobacco plants overexpressing eIF-4A8 containing a histidine tag. Hereby, we could show that indeed eIF-4A8 is modified through phosphorylation. The biological relevance of the phosphorylation of eIF-4A is discussed