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Detection of Protein Structure of Frozen Ancient Human Remains Recovered from a Glacier in Canada Using Synchrotron Fourier Transform Infrared Microspectroscopy

Quaroni, Luca ; Christensen, Colleen R. ; Chen, Becky ; Vogl, Wayne ; Monsalve, Maria Victoria

In: Microscopy and Microanalysis, 2013, vol. 19, no. 3, p. 565-575

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    Titre
    • Detection of Protein Structure of Frozen Ancient Human Remains Recovered from a Glacier in Canada Using Synchrotron Fourier Transform Infrared Microspectroscopy
    Auteur
    • Quaroni, Luca. Paul Scherrer Institut, Villigen-PSI CH-5232, Switzerland
    • Christensen, Colleen R.. College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
    • Chen, Becky. Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    • Vogl, Wayne. Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    • Monsalve, Maria Victoria. Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada V6T 1Z3
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Microscopy and Microanalysis, 2013, vol. 19, no. 3, p. 565-575. Cambridge University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301561
    Summary
    We previously used synchrotron infrared microspectroscopy to describe the biochemical signature of skeletal muscle (biceps brachii) from the frozen ancient remains of a young man. In this current paper, we use light microscopy to assess the state of preservation of cellular components in the trapezius muscle from these same ancient remains and then use mid-infrared analysis at the Canadian Light Source synchrotron facility to further analyze the tissue. We compare spectra between the trapezius samples from the ancient remains and a recently deceased cadaver (control). Infrared spectra indicate preservation of secondary structure, with the α-helix being the principal component, along with triple helical portions of the protein backbone. Our mid-infrared analysis indicates an energy reserve in the skeletal muscle in the ancient remains