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Cyclic-β-glucans of Rhizobium (Sinorhizobium) sp. strain NGR234 are required for hypo-osmotic adaptation, motility, and efficient symbiosis with host plants

Gay-Fraret, Jérémie ; Ardissone, Silvia ; Kambara, Kumiko ; Broughton, William J. ; Deakin, William J. ; Le Quéré, Antoine

In: FEMS Microbiology Letters, 2012, vol. 333, no. 1, p. 28-36

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    Titre
    • Cyclic-β-glucans of Rhizobium (Sinorhizobium) sp. strain NGR234 are required for hypo-osmotic adaptation, motility, and efficient symbiosis with host plants
    Auteur
    • Gay-Fraret, Jérémie. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    • Ardissone, Silvia. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    • Kambara, Kumiko. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    • Broughton, William J.. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    • Deakin, William J.. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    • Le Quéré, Antoine. Laboratoire de Biologie Moléculaire des Plantes Supérieures (LBMPS), Département de Biologie végétale, Sciences III, Universitéde Genève, Genève, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301387
    Summary
    Cyclic-β-glucans (CβG) consist of cyclic homo-polymers of glucose that are present in the periplasmic space of many Gram-negative bacteria. A number of studies have demonstrated their importance for bacterial infection of plant and animal cells. In this study, a mutant of Rhizobium (Sinorhizobium) sp. strain NGR234 (NGR234) was generated in the cyclic glucan synthase (ndvB)-encoding gene. The great majority of CβG produced by wild-type NGR234 are negatively charged and substituted. The ndvB mutation abolished CβG biosynthesis. We found that, in NGR234, a functional ndvB gene is essential for hypo-osmotic adaptation and swimming, attachment to the roots, and efficient infection of Vigna unguiculata and Leucaena leucocephala