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Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Picão, Renata Cristina ; Poirel, Laurent ; Demarta, Antonella ; Silva, Carla Sofia Ferreira ; Corvaglia, Anna Rita ; Petrini, Orlando ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2008, vol. 62, no. 5, p. 948-950

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    Titre
    • Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake
    Auteur
    • Picão, Renata Cristina. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Poirel, Laurent. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Demarta, Antonella. Istituto Cantonale di Microbiologia, Bellinzona, Switzerland
    • Silva, Carla Sofia Ferreira. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    • Corvaglia, Anna Rita. Centre Médical et Universitaire, Université de Genève, Geneva, Switzerland
    • Petrini, Orlando. Istituto Cantonale di Microbiologia, Bellinzona, Switzerland
    • Nordmann, Patrice. Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics', Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Journal of Antimicrobial Chemotherapy, 2008, vol. 62, no. 5, p. 948-950. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301261
    Summary
    Objectives To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland. Methods Isolates presenting MICs of nalidixic acid or ciprofloxacin ≥1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing. Results A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6′)-Ib-cr, blaOXA-1, catB3 and arr-3 gene cassettes. Conclusions These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment