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The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus

Majcherczyk, Paul A. ; McKenna, Therese ; Moreillon, Philippe ; Vaudaux, Pierre

In: FEMS Microbiology Letters, 2006, vol. 255, no. 2, p. 233-239

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    Titre
    • The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus
    Auteur
    • Majcherczyk, Paul A.. Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
    • McKenna, Therese. Waters Corporation, Atlas Park, Manchester, UK
    • Moreillon, Philippe. Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland
    • Vaudaux, Pierre. Service of Infectious Diseases, University Hospital of Geneva, Geneva, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:300627
    Summary
    To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More important changes in MALDI-TOF MS spectra were found with strains differing in methicillin than in teicoplanin resistance. In comparison, very minor or no changes were recorded in pulsed-field gel electrophoresis profiles or peptidoglycan muropeptide digest patterns of these strains, respectively. MALDI-TOF MS might be useful to detect subtle strain-specific differences in ionizable components released from bacterial surfaces and not from their peptidoglycan network