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Involvement of MAPK pathway in TNF-α-induced MMP-9 expression in human trophoblastic cells

Cohen, Marie ; Meisser, Arielle ; Haenggeli, Luise ; Bischof, Paul

In: Molecular Human Reproduction, 2006, vol. 12, no. 4, p. 225-232

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    Titre
    • Involvement of MAPK pathway in TNF-α-induced MMP-9 expression in human trophoblastic cells
    Auteur
    • Cohen, Marie. Department of Obstetrics and Gynaecology, Maternity, Hormone Laboratory, University of Geneva, Boulevard de la Cluse, Geneva, Switzerland
    • Meisser, Arielle. Department of Obstetrics and Gynaecology, Maternity, Hormone Laboratory, University of Geneva, Boulevard de la Cluse, Geneva, Switzerland
    • Haenggeli, Luise. Department of Obstetrics and Gynaecology, Maternity, Hormone Laboratory, University of Geneva, Boulevard de la Cluse, Geneva, Switzerland
    • Bischof, Paul. Department of Obstetrics and Gynaecology, Maternity, Hormone Laboratory, University of Geneva, Boulevard de la Cluse, Geneva, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Molecular Human Reproduction, 2006, vol. 12, no. 4, p. 225-232. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:300011
    Summary
    The aim of this article was to investigate the signalling pathways involved in metalloproteinase-9 (MMP-9) expression induced by tumour necrosis factor-α (TNF-α) in first-trimester trophoblastic cells. TNF-α-induced MMP-9 expression, secretion and activity were completely blocked by stress-activated protein kinase/jun kinase (SAPK/JNK) and Erk inhibitors (SP600 125 and U0126 respectively) but not by p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203 580 and SB202 190). Stimulation of HIPEC 65 cells with TNF-α caused phosphorylation of JNK and extracellular signal-regulated kinase 1/2 (Erk1/2), with a peak after 20 min of treatment. Transcription factors nuclear factor-κB (NF-κB) and activator protein 1 (AP-1)-binding site were identified as the cis-elements involved in TNF-α activation as determined by electromobility shift assays. TNF-α-induced transactivation of NF-κB was inhibited by U0126, whereas TNF-α-induced transactivation of AP-1 was inhibited by SP600 125. Taken together, these results indicate that in trophoblastic cells, TNF-α probably activates two different pathways leading to MMP-9 expression: (a) Erk1/2 pathway which in turn initiates NF-κB activation and (b) SAPK/JNK pathway that activates AP-1