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Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island

Albonico, Marco ; Rinaldi, Laura ; Sciascia, Sonia ; Morgoglione, Maria E. ; Piemonte, Monica ; Maurelli, Maria P. ; Musella, Vincenzo ; Utzinger, Jürg ; Ali, Said M. ; Ame, Shaali M. ; Cringoli, Giuseppe

In: Transactions of The Royal Society of Tropical Medicine and Hygiene, 2013, vol. 107, no. 8, p. 493-501

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    Titre
    • Comparison of three copromicroscopic methods to assess albendazole efficacy against soil-transmitted helminth infections in school-aged children on Pemba Island
    Auteur
    • Albonico, Marco. Department of Biology, University of Torino, 10122 Torino, Italy
    • Rinaldi, Laura. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via della Veterinaria 1, 80137 Naples, Italy
    • Sciascia, Sonia. Department of Biology, University of Torino, 10122 Torino, Italy
    • Morgoglione, Maria E.. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via della Veterinaria 1, 80137 Naples, Italy
    • Piemonte, Monica. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via della Veterinaria 1, 80137 Naples, Italy
    • Maurelli, Maria P.. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via della Veterinaria 1, 80137 Naples, Italy
    • Musella, Vincenzo. Department of Health Sciences, University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy
    • Utzinger, Jürg. Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, PO Box, CH-4002, Basel, Switzerland
    • Ali, Said M.. Public Health Laboratory-Ivo de Carneri, Pemba, Tanzania
    • Ame, Shaali M.. Public Health Laboratory-Ivo de Carneri, Pemba, Tanzania
    • Cringoli, Giuseppe. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via della Veterinaria 1, 80137 Naples, Italy
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Transactions of The Royal Society of Tropical Medicine and Hygiene, 2013, vol. 107, no. 8, p. 493-501. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:298965
    Summary
    Background The diagnostic accuracy of three faecal egg count techniques (Kato-Katz, McMaster and FLOTAC) to assess albendazole efficacy against soil-transmitted helminth (STH) infections was compared. Methods The study is registered with Current Controlled Trials [identifier: ISRCTN90088840]. During September-November 2009, 304 school-aged children on Pemba Island, Tanzania, were screened and those infected with Ascaris lumbricoides, hookworm or Trichuris trichiura were treated with a single dose of albendazole (400 mg). Twenty-one days post-treatment, children provided a single stool sample which was examined using the same diagnostic methods. All stool samples were divided into two aliquots and one was fixed in 5% formalin and examined using FLOTAC and McMaster approximately 6 months after collection. Results Using fresh stool samples, comparable prevalences were demonstrated for the three methods at baseline (90-92.2% for T. trichiura, 41.1-52.8% for hookworm, 32.9-37.2% for A. lumbricoides); FLOTAC was the most sensitive method at baseline and follow-up. Albendazole showed high cure rate (CR) against A.lumbricoides (90-97%), moderate CR against hookworm (63-72%) and very low CR against T.trichiura (6-9%), regardless of the technique used. Egg counts (eggs per gram) at baseline were similar for A. lumbricoides and for hookworm among the three methods, and higher using McMaster and Kato-Katz compared with FLOTAC for T. trichiura. All methods were similar for hookworm and A. lumbricoides egg reduction rate (ERR) estimation, but Kato-Katz indicated a significantly higher ERR than McMaster and FLOTAC for T. trichiura. Preserved stool samples revealed consistently lower FECs at baseline and follow-up for all STHs. Conclusion Further development and validation of standard protocols for anthelminthic drug efficacy evaluation must be pursued